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Authors
Shiv Kumar Yadav
1,
Dharmendra Kumar Gangwar
1,
Jarnail Singh
1,
Chiranjeev Kumar Tikadar
1,
V. Vinoth Khanna
1,
Sudha Saini
1,
Sunny Dholpuria
1,
Prabhat Palta
1,
Radhey Shyam Manik
1,
Manoj Kumar Singh
1,
Suresh Kumar Singla
1 Affiliations
1 Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal-132001, Haryana, IN
Source
Veterinary World, Vol 10, No 5 (2017), Pagination: 498-504
Abstract
Separation of X- and Y-chromosome bearing sperm has been practiced for selection of desired sex of offspring to increase the profit in livestock industries. At present, fluorescence-activated cell sorter is the only successful method for separation of X- and Y-chromosome bearing sperm. This technology is based on the differences in DNA content between these two types of sperm and has been commercialized for bovine sperm. However, this technology still has problems in terms of high economic cost, sperm damage, and lower pregnancy rates compared to unsorted semen. Therefore, an inexpensive, convenient, and non-invasive approach for sperm sexing would be of benefit to agricultural sector. Within this perspective, immunological sperm sexing method is one of the attractive choices to separate X- and Y-chromosome bearing sperm. This article reviews the current knowledge about immunological approaches, viz., H-Y antigen, sex-specific antigens, and differentially expressed proteins for sperm sexing. Moreover, this review also highlighted the different methods for identification of X- and Y-sperm.
Keywords
Differentially Expressed Proteins, H-Y Antigen, Sex Specific Proteins, Sperm Identification, Sperm Sexing.
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